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BIOPROCESS

A group web-blog by MB0606 encompassing our experiences in learning how to produce functional GFPs in bacteria.

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Wednesday, November 28, 2007
Day Three Part I
11:55:00 AM

Day 3, Part I

Experiment 3: Inoculation, Fermentation and Monitoring

During the third day, we carried out sampling, which was essentially done to monitor the growth of cells as well as the rate of product formation.

Objectives
1. To carry out scale-up fermentation process for GFP protein
2. To monitor cell growth and product formation through manual sampling and computer data logging

Part II of monitoring cell growth and product formation (sampling)

1. 10ml of blank sample were taken before inoculation and then one sample (10ml) every hour.
2. The fermentation broth was harvested after 24 hours of fermentation, and 10ml of culture was aliquot into a sterile, disposable test tube. The absorbance of the sample will be taken and the growth curve will be drawn out.


There are four sections to Day 3:

You Are Here: Part I (Procedures)



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